Each calibration standard solution is prepared based on the previous calibration standard. The process involves taking a portion of the previous standard and diluting it with the solvent to obtain the next calibration standard. The errors introduced with each successive dilution drops proportionately with the solution concentration. Preparing a series of calibration standards by this method reduces the amount of required time. Most calibration standards span a large range of concentrations, so the accuracy of the calibration standard prepared increases.

Direct dilution is the process by which Echo Liquid Handlers generates the IC 50 curve, transferring precise droplets of solution directly to individual assay wells, without tips. This straightforward process can generate a 12-point IC 50 curve from a stock solution and two intermediate dilutions, improving reliability and data quality.

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Calibrations Solutions More Evenly Spaced. The dilution factor chosen for the series of calibration standards is achievable by using serial dilution. The progression of calibration standard concentration is always a geometric series.

Consider the example of making the first standard at 1/3 the concentration of the known, the next calibrant would be 1/9th the concentration of the known and the following two calibrants formed are 1/27th and 1/81st. This becomes a much greater advantage when the span of the calibration standards must cover several orders of magnitude in concentration.